The aim of this paper is to investigate the threshold temperatures and chilling accumulation for breaking the natural dormancy of blueberries in accordance with meteorological standards, and the study subject is the rabbit-eye blueberries in Majiang. By using the phenological observations and concurrent daily temperature data and the curve-fitting method, this paper determines the initial and final dates of threshold temperatures at 1℃ intervals from 1℃ to 13℃. The active accumulated temperature method is adopted to calculate the chilling accumulation between the final and initial dates of each threshold temperature. Standard deviation (SD) and coefficient of variation (CV) are obtained to determine the stability of each chilling accumulation. The threshold temperature corresponding to the chilling accumulation with the smallest absolute difference between CV and SD, combined with an 80% validation rate, is identified as the optimal dormancy threshold for the Majiang rabbit-eye blueberries.‌ The results indicate that the suitable dormancy temperature for blueberries has interannual variability, increasing with rising averate temperatures during the dormancy period. The optimal dormancy temperature remains relatively stable and shows no significant correlations with average dormant-phase temperatures, effective upper cold thresholds, or the most effective upper cold thresholds. For 80% of the study years, the optimal dormancy temperature is 5℃, corresponding to a chilling accumulation of approximately 170℃· corresponding to a chilling ccumulation can be observed when the daily average temperature during dormancy is ≤ e℃.‌ Therefore, the most effective dormancy temperature range for Majiang rabbit-eye blueberries is 0.0 ancy℃, with a chilling accumulation requirement of 441.1℃·equwhich is calculated based on the accumulated temperature of daily averages within the 0.0–10.0℃ range during dormancy. These findings establish a meteorologically driven scientific framework that can support precise dormancy regulation in subtropical blueberry cultivation.