Abstract: [Purpose] During the freezing and rainy weather at the end of February 2024, the seedlings of radix pseudostellariae in Niudachang and Baiduo base in Shibing County experienced varying degrees of freezing damage. [Methods] To identify the meteorological causes of freezing damage, this study conducted a statistical analysis of temperature, precipitation, and other data from meteorological observations near the survey site. The freezing damage indicators during the seedling stage of the radix pseudostellariae were obtained based on the comparative analysis of different degrees of freezing damage. [Results] Results showed that radix pseudostellariae seedlings had strong resistance to low temperature. However, freezing damage would occur when the low-temperature freezing weather lasts long. The degree of freezing damage increased with the increase of ice cover time, the decrease of minimum temperature, the increase of negative accumulated temperature, and the increase of precipitation. The radix pseudostellariae seedings were slightly freeze-damaged in freezing and rainy weather when the daily minimum temperature was maintained between -4 to -5 ℃ for three consecutive days, the negative accumulated temperature reached 12.5 ℃ per day, and the ice was continuously covered for 2-3 days. Obvious freeze damage occurred when the daily minimum temperature was maintained between -5 to -6 ℃ for three consecutive days, the negative accumulated temperature reached 18 ℃ per day, and the ice was continuously covered for 3-5 days. Severe freeze damage occurred when the daily minimum temperature lowered than -6 ℃ for three consecutive days, the negative accumulated temperature exceeded 18 ℃ per day, and the ice was continuously covered more than 5 days. [Conclusion] The above meteorological indexes are based on case analysis, which are suitable for the planting base of radix pseudostellariae in Qiandongnan, Guizhou, and have certain guiding significance for the meteorological service of the radix pseudostellariae production.