Abstract: This paper used data for one year from the observation of Negative Oxygen Ions (NOIs) in Jingde county, Tuershan Park and Luxi Village Tea Garden were chosen as two different ecological environments of Urban Park, Alpine Tea Garden, compared and analyzed the characteristics of NOIs concentrations between Urban Park and Alpine Tea Garden and the correlation with meteorological factors. The results showed that: ①The concentrations of NOIs were significantly higher in Alpine Tea Garden than those in Urban Park. ②The concentrations of NOIs were the highest in Summer, the lowest in Winter, followed by Spring and Autumn, and reached the highest in June. ③The daily variation of NOIs concentrations in these two regions showed a trend of “single peak and single valley”, with the peak value appeared in the morning and the valley value appeared in the noon, the concentrations of NOIs were higher from night to morning, and the daily range of NOIs concentrations in Urban Park were smaller than that in Alpine Tea Garden. ④the daily variation of concentrations of NOIs were significantly negatively correlated with air temperature and wind speed. ⑤The concentrations of NOIs were highest in rainy day, followed by sunny day, and lower in overcast day and cloudy day, the concentration of NOIs increased significantly under rainstorm conditions.