The spatial resolution of FY-3B TOU total ozone product is 50 km，and it is necessary to obtain high-resolution, more accuracy and reliability total ozone interpolation data, when carrying out small area or refined ozone studies.Conventional data interpolation methods do not consider the influence of short wave radiation and altitude on the total ozone amount of TOU, and the interpolation data obtained are not highly referential. This paper introduces the multiple regression interpolation method of TOU. The method adopts the programming method to dynamically analyze the correlation between TOU and shortwave radiation, altitude, establish a single regression model, establish a multiple regression model according to the structure of the single regression model, and then interpolate TOU through the multiple regression model. The OMI ozone total data was used to verify the interpolation results, and the verification results showed that the interpolation using multiple regression algorithm was feasible. And the interpolation method and program design have certain reference significance for other similar data interpolation and analysis.