In order to study the effect of low temperature on the physiological characteristics of strawberry, this study used strawberry "beauty" as the research object, and carried out a low temperature single factor control test in the Venlo experimental greenhouse of Nanjing University of information engineering from August 2016 to March 2017 to systematically measure various indexes of Strawberry Leaves under low temperature treatment. The results showed that: (1)The longer the stress, the overall trend of chlorophyll decreased. In particular, the content of chlorophyll a decreased significantly after treatment at 0℃ for 7 days. (2) At the same temperature, the soluble protein content in strawberry leaves increased first and then decreased with the extension of time, and each treatment was higher than those of ck Control group 10-110 percentage points. At the same time and at different temperatures, the soluble protein content of 8℃ was the highest, and the soluble protein content of ck was the lowest. (3)At the same temperature with the extension of time, the change of soluble sugar showed a downward trend, the treatments were significantly higher than ck 23-57 percentage points except the treatment for 7 days. At the same time and at different temperatures, the higher the temperature, the higher the content, and the treatments were higher than CK group except the treatment for 7 days. (4) At high temperature, photosynthetic rate is higher, light compensation point is significantly larger, and light saturation point is generally higher.